Journal: bioRxiv

Article Title: Selective IL-27 production by intestinal regulatory T cells permits gut-specific regulation of Th17 immunity

doi: 10.1101/2023.02.20.529261

Figure Lengend Snippet: (A) UMAP plots of intestinal Treg cell clusters, colored by cluster identity. (B) Heatmap of top 10 differently expressed genes between each intestinal Treg cell clusters. (C) Percentage of cells within each intestinal Treg cell cluster from mice with or without C. rodentium infection. Violin plots of (D) Il27 , (E) Ebi3 , (F) Cd83 , and (G) Tcf7 in different intestinal Treg cell clusters from C. rodentium- infected mice. (H) FACS analysis of intestinal Treg cell clusters based on the expression fo CD83 and TCF1. (I) Representative of FACS profiles with gating strategy for isolating different intestinal Treg cell subsets. qPCR analyses for the expressions of (J) Il27 , (K) Ebi3 , and (L) Il10 in different Treg cell subsets in spleen or LI LP from C. rodentium- infected mice. Each symbol represents an individual mouse, and the bar represents the mean. Data are pooled from two independent experiments. n.s., not significant; *, P < 0.05; ***, P < 0.001; ****, P < 0.0001.

Article Snippet: Cells were stained with Ghost Dye Red 780 (catalog 13-0865-T100; Tonbo Biosciences), followed by surface and intracellular antibody staining for CD4 (catalog 45-0042-82; Thermo Fisher Scientific); Ki67 (catalog 51-5698-82; Thermo Fisher Scientific); CD62L (catalog 12-0621-82; Thermo Fisher Scientific); CD8α(catalog 25-0081-82; Thermo Fisher Scientific); CD44 (catalog 48-0441-80; Thermo Fisher Scientific); IFNγ (catalog 25-7311-82; Thermo Fisher Scientific); IL-17A (catalog 48-7177-82; Thermo Fisher Scientific); CD25 (catalog 12-1522-82; eBioscience); CD83 (catalog 121508; Biolegend); TCF1 (6709S; Cell Signaling); ST2 (catalog 46-9335-82; eBioscience); and Foxp3 (catalog 53-5773-82; Thermo Fisher Scientific) at the manufacturer’s recommended concentrations.

Techniques: Infection, Expressing

Journal: Journal of Functional Foods

Article Title: Bilberry anthocyanins attenuate mitochondrial dysfunction via β-catenin/TCF pathway in Alzheimer’s disease

doi: 10.1016/j.jff.2023.105827

Figure Lengend Snippet: Fig. 8. The protective effect of BAs against Aβ42-induced neurotoxicity in HT22 cells was abrogated by ICG-001 and LF3. A-B. Changes in cell viability following treatment with 5 µM Aβ42 alone or in combination with 100 μg/mL BAs ± 4 µM ICG-001 (A) or ± 2.5 µM LF3 (B) for 24 h. C. Changes in ATP levels based on luciferin-luciferase ATP assay (n = 3). D. Changes in mitochondrial membrane potential based on flow cytometry analysis (labeled with JC-1) in HT22 cells treated with 5 µM Aβ42 alone or in combination with 100 μg/mL BAs ± 4 µM ICG-001 (n = 3). A representative data set is shown. E. Western blot analysis of the proteins related to mitochondrial homeostasis in HT22 cells treated with 5 µM Aβ42 alone or in combination with 100 μg/mL BAs ± 4 µM ICG-001. F. HT22 cells were treated with 5 μM Aβ42 ± 100 μg/mL BAs or 2.5 µM ICG-001 for 24 h, and then the cellular protein extracts were immunoprecipitated with an anti-β-catenin antibody. The immune-precipitates were analyzed by Western blotting for active β-catenin and TCF7 protein levels.

Article Snippet: The anti-Mfn2 (#A12771), anti-Fis1 (#A5821), anti-Opa1 (#A9833) and anti-TCF7 (#A3091) antibodies were obtained from ABclonal (Boston, MA, USA); the anti-non-phospho-β-catenin (active) (#8814) and anti-β-catenin (#8480) antibodies were from CST (Danvers, MA, USA).

Techniques: Luciferase, ATP Assay, Membrane, Flow Cytometry, Labeling, Western Blot, Immunoprecipitation